Video of Colossal Squid Dissection

TPOTH

Vampyroteuthis
Registered
#2
:shock: Holy shi....*ahem*... Great stuff.
I'm also truly amazed by the tiny size of the statolith, I dissected some O. cyanea and only the smallest individuals (below 400g...granted that might not be a true indication of age as weight/age ratio is very much dependent on the feeding rate/environment...still O. cyanea is reaching 2kg in less than a year) had statoliths that small so are we to assume that this Colossal Squid is a young one?
I must remember to hassle Steve and Kat about the result of the ring counting...
:squidaut:
Any computer wiz know a way to download and save that video?

TPOTH
 

Jean

Colossal Squid
Supporter
#3
I think most squid statoliths are comparatively small in relation to the squid size (steve?????? Kat????????) I too would love to hear the outcome of the counts

J
 

Jean

Colossal Squid
Supporter
#6
Hey Kat, Steve....did you ever get a putative age on Messie??? It came up at work when we were discussing the content of some display boards for the new squid display. What's your feeling about max age for messie.......archie..... I had a mature Nototodarus at 206 days old.....d'ya reckon 3-4 years for these big ones......older????

Cheers

J
 

main_board

Vampyroteuthis
Supporter
#7
Great questions Jean, I'd love to know too. I believe Steve said in the doco that one layer of stuff (some sort of calcium compound?) was deposited on the statolith each day. How do we know this? Anyone know of a reference to this? I'm just curious as that seems pretty frequent and also a pretty specific ratio considering we are just now getting "good" at aquaculture of these cephs. Now we can raise a group of squid and definitely know the ages of individuals and therefore relate that to the number of rings on their statoliths and from that develop some relationship. Bad sentence, but hopefully you get the meaning of it. Has some one done this exact thing and that's how we know? Just curious.

Cheers!
 

Feelers

Vampyroteuthis
Registered
#8
Whats a statholith for?
I remember in plant biology its small granules within a seed that fall to the bottom of the cell due to gravity and tells the seed which way is up so it grows in the right direction.

And how do the deep squid keep time?(to add to the statholith) Wouldnt they be on a free run period? Does anyone know if its 24 hours long?
 

main_board

Vampyroteuthis
Supporter
#9
They are similar in function to that of the plants' statolith. They tell the squid its orientation in the water relative to gravity and also, I believe, acceleration and maybe even velocity.

http://www.tonmo.com/forums/showthread.php?t=2511&goto=nextoldest

Some similar ideas are discussed there. The bottom few posts would be the ones of interest.

I just took a brief glimpse through Nesis to learn some more about statoliths and he says that often after a certain age the squid discontinues adding layers to the statoliths. Therefore the statoliths of adult squid could be similar to younger squid as well. They just don't make it easy on us, do they?!?!?!

Cheers!
 

monty

Colossal Squid
Staff member
Supporter
#10
main_board said:
They are similar in function to that of the plants' statolith. They tell the squid its orientation in the water relative to gravity and also, I believe, acceleration and maybe even velocity.
physics nitpick: the statocyst certainly measures acceleration, gravity being a special case of that, but it has no way to measure velocity, for the same reason that if you're in a moving vehicle, nothing "feels" different at a constant speed than it does when you're standing still-- there's nothing to measure unless you can see or feel the medium you're travelling through (the road, the water, or the air).
 

tonmo

Titanites
Staff member
Webmaster
Moderator
#11
Amazing! I had never seen that video before -- thanks Jean for resurrecting this year+ old thread. That was awesome! Can't believe I never saw that.

Steve / Kat -- please demystify that statolith for us! I'm with Phil -- how would you go about finding that thing?
 

Tintenfisch

Architeuthis
Staff member
Moderator
#14
That was in... ummm... late 2003? Will have to do some searching for the statolith (ironic that it may be more difficult to find now that we've taken it *out* ... oh well...). Steve may have sent it off to a colleague in Tasmania, or it may be waiting to go.
In any case, the statocysts are located within the ventral part of the cartilaginous cephalon (kind of a skull), so you make one horizontal cut, and then do very thin slices until you find the statocyst. Then it's just a matter of picking out the statolith before it's washed away in a tide of slime. Simple, really... :wink:
 

chrono_war01

Colossal Squid
Supporter
#15
Oh Ye Gods and Garlic Dumplings, it is HUGE!:bugout:

Great Stuff, Steve and Kat.

I'm jumping for joy and my classmates are giving me werid glances now.:wink:
 

Jean

Colossal Squid
Supporter
#17
main_board said:
Great questions Jean, I'd love to know too. I believe Steve said in the doco that one layer of stuff (some sort of calcium compound?) was deposited on the statolith each day. How do we know this? Anyone know of a reference to this? I'm just curious as that seems pretty frequent and also a pretty specific ratio considering we are just now getting "good" at aquaculture of these cephs. Now we can raise a group of squid and definitely know the ages of individuals and therefore relate that to the number of rings on their statoliths and from that develop some relationship. Bad sentence, but hopefully you get the meaning of it. Has some one done this exact thing and that's how we know? Just curious.

Cheers!
The deposition rate has been verified for some species of squid by either injecting a fluorescent dye or immersing the animal in it. The dye (usually Calcein or Tetracycline) is taken up into the statolith at the same rate as calcium so you get a fluorescent ring when you look at it under UV light. So if you know the date the dye was intoduced and the date the animal died then you count up the number of rings inbetween and relate them with a time period. Most often in squid it seems to be daily. In the big species (Messie et al) and some small oceanic ones the periodicity of the rings is inferred by their resemblance to verified species because of the difficulty in keeping the animals live in the lab.

Here's a couple of refs but there are HEAPS more. This is also a technique used for aging fish and shellfish such as clams!

Jackson, GD; Forsythe, JW. 2002. Statolith age validation and growth of Loligo plei (Cepahlopoda: Loliginidae) in the north-west Gulf of Mexico during spring/summer Journal of the Marine Biological Association of the United Kingdom [J. Mar. Biol. Assoc. U.K.]. Vol. 82, no. 4, pp. 677-678.

Dimmlich, WF; Hoedt, FE. 1998. Age and growth of the myosid squid Loliolus noctiluca in Western Port, Victoria, determined from statolith microstructure analysis. Journal of the Marine Biological Association of the United Kingdom [J. Mar. Biol. Assoc. U.K.]. Vol. 78, no. 2, pp. 577-586.

Lipinski, M. 1986. Methods for the Validation of Squid Age from Statoliths Journal of the Marine Biological Association of the United Kingdom [J. Mar. Biol. Assoc. U.K.]. Vol. 66, no. 2, pp. 505-526.

Dawe, EG; O'Dor, RK; Odense, PH; Hurley, GV. 1985.Validation and application of an ageing technique for short-finned squid (Illex illecebrosus ). Journal of Northwest Atlantic fishery science [J. NORTHW. ATL. FISH. SCI.]. Vol. 6, no. 2, pp. 107-116.




Tintenfisch said:
Steve may have sent it off to a colleague in Tasmania,
Would that be George????

Cheers

J
 

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