Nice SEMs

Tintenfisch

Architeuthis
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Moderator
#1
Hmmm, no new posts in the onychoteuthid section since late 2008 - we can't have that! :wink: I've been sorting out all of the SEM stubs I've accumulated over the last 5 years and getting them, and the images themselves, ready to return to the museums they were loaned from. Thought the community might also enjoy seeing some of these images.

Here are some radulae:
 

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DWhatley

Cthulhu
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#12
Kat, do you know the magnification levels on the radula and suckers? Seeing the "plunger" effect more or less as a functional sequence is neat. Any more "how it works" pictures?
 

cuttlegirl

Colossal Squid
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#13
D,

In the pictures there is a measurement bar in microns. A micron is 1000th of a millimeter. Does this help? In the top left picture of the radula, the bar is 200 microns - that is about the width of three of the middle teeth, so the width of three of the middle teeth would be 0.2 millimeters. On the bottom left image of the hooks, the width is 1 millimeter, so the total length of that hook would be about 1.5 millimeters.
 

DWhatley

Cthulhu
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Moderator
#14
A little after I enlarged the photos to read them but I am afraid I don't get the spacial feel for how many times reality I am looking at when somthing is stated that way. If the bar in the key is 200 x .001 or .2 mm I can look at a tape and guestimate 1/5 th of a mm but that does not help me visuallize the magnification level - just did not do bio in college (night classes meant astromomy even though I wanted to take biology) and have not even toyed with a microscope since I was a kid.
 

cuttlegirl

Colossal Squid
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#15
In the first photo of the radula, it says 1600x magnified, but that is not taking into account the size of the image that you are viewing. If you enlarge the photos, then you are magnifying it even further. Are you confused yet?
 

DWhatley

Cthulhu
Staff member
Moderator
#16
It does help a lot with spacial imagery to know the magnification. I can't tell the actual size of what I am looking at but I can get prospective. If you magnify your thumb 1600 X's you can see finer details so it is the amount of detail with relation to normal viewing that knowing the magnification level helps intuitively understand.

Hey, I still prefer an analog clock because I can see how late I am :roll: because it displays a picture of an hour's worth of time and the relationship of the current time and when I was supposed to be somewhere. :old:
 

Tintenfisch

Architeuthis
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Moderator
#17
cuttlegirl;136281 said:
Yeah, it is hard to stop breathing while mounting specimens for SEM...
Yeah... don't breathe, don't sneeze (almost as difficult for me as not breathing for most people :roll:), don't drop it onto the sticky surface upside down, don't squeeze too hard otherwise you get tweezer-marks, don't let your hands shake... actually it's a wonder I got any of these pics at all. :roll:

Here are some more. These are tentacle clubs of paralarval onychoteuthids. Sorry that some have mags on them and some don't - I usually don't include them in the legend because journals don't want them, due to image resizing changing the mag. Scale bars are much preferred even if they make visualization a little harder for some.
 

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Tintenfisch

Architeuthis
Staff member
Moderator
#18
And here are some 'how it works' photos, kind of.

1 - a normal adult arm sucker in Onychoteuthis
2 - a tentacular sucker in the middle of developing into a hook, in paralarval Onychoteuthis
3 - the 'back' (interior/aboral side) of an exposed paralarval tentacular sucker ring - the smooth rounded part in the foreground would normally be set into the sucker musculature
4 - a tentacular sucker in the middle of developing into a hook, in paralarval Onykia
 

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Jean

Colossal Squid
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#20
Tintenfisch;136367 said:
Yeah... don't breathe, don't sneeze (almost as difficult for me as not breathing for most people :roll:)
I can relate, I have sneezed an entire tray of statoliths into oblivion!
 

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