Cephalopod Studies for Human Wellbeing

DWhatley

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#41
Iron Profile of Octopus Hubbsorum (Cephalopoda: Octopodidae) for Enrichment of the Mexican Diet
Palacios-Abrantes Juliano , Melo-Ruiz Virginia , Diaz-Garcia Rafael, Gazga-Urioste César, Urbano Brian 2016 (full paper)

Abstract: FAO (Food and Agriculture Organization of the United Nations) declared that 12% of the world’s population is undernourished while in Mexico the number is around 5%. Minerals are essential for the daily diet; iron (Fe) is an important mineral on human welfare because it has a main role on the function of the immune and central nervous systems, among others. Most Mexicans fulfill their iron requirements from vegetables, which are poorly absorbed; nevertheless, in coastal populations, where seafood-rich in iron- is available, it is not consumed on a regular diet. The aim of this work was to analyze minerals, focusing on the iron content of Octopus hubbsorum B (Cephalopoda: Octopodidae), an animal of the Mexican Pacific coast. Three specimens were analyzed, two from the north and one from central Pacific Ocean. Data obtained for the central Pacific Octopus was 0.008% and data for the North Pacific were 0.008% and 0.011%, repectively. In conclusion, the supply of iron for the Mexican diet provided by Octopus hubbsorum B is significant, therefore is recommended to consume more of this resource, to diminish iron deficiency, a world-wide problem, and to improve human health.
 

DWhatley

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#42
Researchers find medical application for squid skins
Taiwan Times, Wu Hsin-tien and Jake Chung 2/9/2017
Discarded squid skins can be refined into an anti-inflammatory liposome with applications for humans and pets, the Council of Agriculture’s Fisheries Research Institute said.

After fishermen skin squids and process them into slips, rings and dried products, the skins are usually discarded, institute assistant researcher Kao Yi-feng (高翊峰) said, adding that the nation’s fishermen catch nearly 260,000 tonnes of squid and generate roughly 27,000 tonnes of squid skins each year.

The institute has discovered that phospholipids can be extracted from skins after they have set, which after refinement could be processed into the liposome, he said.

After combination with a low-temperature, high-frequency ultrasound process, the institute was able to further process the liposome into pressurized sprays, similar to inhalers used by asthmatics, he added, adding that after inhalation, the body processes the liposome, which causes an anti-inflammatory reaction.

Applications for the squid skin liposome include easing pain or discomfort after cosmetic surgeries or for patients with large burns, Kao said, adding that as it is a unique compound prescription, it has yet to meet the single prescription legal requirement.

At least 10 years of research would be needed to develop the liposome into a medication, he said, adding that from the start, research has focused more on a healthcare product or for use on pets. ...
 

DWhatley

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#43
Isolation and partial characterization of collagen from outer skin of Sepia pharaonis (Ehrenberg, 1831) from Puducherry coast
Jayalakshmi Krishnamoorthia,Pasiyappazham Ramasamya, Vairamani Shanmugama, Annaian Shanmugama 2017 (ScienceDirect open access)
Abstract
Type I collagen from outer skin of Sepia pharaonis was extracted and partially characterized. Yield of Acid Soluble Collagen (ASC) and Pepsin Soluble Collagen (PSC) were calculated as 1.66% and 3.93% and the total protein content of ASC and PSC were found as 18.4% and 48.6%. FT-IR spectrum of ASC and PSC recorded 12 and 14 peaks, respectively. 1H-NMR spectrum of ASC showed singlets at 1.23 ppm, 3.1 ppm, 3.55 ppm and 3.7 ppm and PSC at 1.23 ppm and 2.08 ppm. The molecular weight for ASC was calculated as 102 kDa and for PSC as 110, 108 and 102 kDa through SDS-PAGE. Differential Scanning Calorimetry (DSC) results supported that PSC withstand high thermal stability (82.85 °C) than ASC (73.13 °C). Higher denaturation temperature with high molecular weight well support the property of type I collagen from skin of S. pharaonis and it could be used as another potent source for the extraction of collagen.
 

DWhatley

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#44
In vitro preparation and assessment of radical reducing peptide from Octopus aegina using digestive proteases
Sekar Sudhakar, Rasool Abdul Nazeer 2017 (subscription ScienceDirect)

Antioxidant peptides protect biological macromolecules against radical damages. The use of these peptides was evaluated using free radicals scavenging assays [2,2-diphenyl-1 picrylhydrazyl (DPPH) and hydroxyl] with the help of UV-visible and electron spin resonance (ESR) spectroscopy methods. The Octopus aegina mantle protein were tested upon hydrolysis using gastrointestinal enzymes up to 12 h, where pepsin hydrolysate exhibited superior properties (DPPH: 44.39 ± 0.67% and hydroxyl: 38.84 ± 1.07%) compared with trypsin and α-chymotrypsin. Consequently, the antioxidant activity of the purified hydrolysate increased on a successive purification, and the peptide sequence was determined to be 368.9 Da with Gly-Glu-Tyr amino acids. Tripeptide exerted free radical scavenging efficiency in DNA damage, lipid peroxidation and cellular destruction (MCF7 cells) under stress condition. The results obtained with octopus antioxidant peptide suggested its role as an adjunct in food and pharmaceutical industries.
 

DWhatley

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#45
Antibacterial activity of methanol and water extract of Loligo duvauceli ink
Nisha N, Dr. S Suja 2017 (short pdf paper)

4. Conclusion In this studies the antibacterial activity of Loligo duvauceli ink in methanol and water extract for E. coli and S. aureus were tested, compared to methanol extract water have more antibacterial activity. The studies related to antioxidant and anticancer activity of Loligo duvauceli ink are going on. Thus this study recommends the use of squid ink has available biopharmaceutical product with antibacterial activity.
 

DWhatley

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#46
A Potential Adjuvant Agent of Chemotherapy: Sepia Ink Polysaccharides
Fangping Li, Ping Luo, Huazhong Liu 2018 (Marine Drugs, Full Paper)

Abstract
Sepia ink polysaccharide (SIP) isolated from squid and cuttlefish ink is a kind of acid mucopolysaccharide that has been identified in three types of primary structures from squid (Illex argentinus and Ommastrephes bartrami), cuttlefish Sepiella maindroni, and cuttlefish Sepia esculenta ink. Although SIP has been proved to be multifaceted, most of the reported evidence has illuminated its chemopreventive and antineoplastic activities. As a natural product playing a role in cancer treatment, SIP may be used as chemotherapeutic ancillary agent or functional food. Based on the current findings on SIP, we have summarized four topics in this review, including: chemopreventive, antineoplastic, chemosensitive, and procoagulant and anticoagulant activities, which are correlative closely with the actions of anticancer agents on cancer patients, such as anticancer, toxicity and thrombogenesis, with the latter two actions being common causes of death in cancer cases exposed to chemotherapeutic agents.
 

DWhatley

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#47
Exploring the Chemical Composition and Anticancer Potential of Oil from Squid (Loligo duvauceli) Liver Waste from Fish Processing Industry
Meivelu Moovendhan, P. Seedevi, S. Vairamani, A. Shanmugam 2018 (Springer subscription)

Abstract
The present study was aimed to evaluate the nutritional fact and anticancer effect of liver oil extracted from the squid Loligo duvauceli. The ash and moisture content was noted as 3.21 and 2.13% whereas the total protein, lipid and carbohydrate, recorded 23.44, 21.23 and 2.13% respectively. The water and fat soluble vitamins and minerals were found in good level. The lipid profile was noted similarly to cod liver oil at the same time the good cholesterol was found in high level. Ten fatty acids were found especially DHA, EPA, omega-3 in high level. The essential amino acids were recorded at high level whereas Non-essential amino acids, moderate level. The anticancer ability of oil against lung cancer cell line (A549) showed better activity, up to 70% inhibition was noted at 960 µg/ml with the CTC50 of 260 µg/ml. In addition to this, there cytotoxicity effect was seen in Vero (normal) cell lines. The present study concludes that the L. duvauceli liver oil is a good source of nutrition and provides good health benefits to human.
 

tonmo

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#48
love this thread, D!
 

DWhatley

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#49
Octopus Algorithm for Wireless Personal Communications
Anwesha Mukherjee. Debashis De 2018 (subscription Wireless Personal Communications via Springer)

Abstract
Bio-inspired computation has opened a new window towards the solution of different computational problems. In this article we propose octopus algorithm for the first time based on the arm movement of octopus during feeding and then applied the algorithm in the field of mobile network to achieve power optimization. The mathematical model of octopus arm movement is presented in this article. Three power-efficient applications for recovery management and offloading in fifth generation mobile network are proposed based on the octopus algorithm. Simulation results prove that use of octopus algorithm optimizes the power consumption in mobile network.
 

DWhatley

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#50
Preliminary screening of the antibacterial effect of the pigmented squid ink extracts against methicillin resistant Staphylococcus aureus (MRSA)
GAS Smiline, PJ Vijayashree - 2018 PDF

The present investigation is to assess the antibacterial property of the squid ink extracts from Loligo duvauceli against methicillin resistant Staphylococcus aureus [MRSA]. MRSA strain was isolated and was characterized by standard microbiological methods. Tenotypic detection of mecA gene was don by PCR. Isolated strain was further subjected to antibacterial bioassay with the crude extracts of squid ink obtained using various solvents. Hexane extract scores a promising antimicrobial effect against MRSA, with trace activity by ethyl acetate extract and no activity by other solvent extracts. MIC value was determined as 2.5 mg/ml
 

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