View Full Version : Translucent squid - how did this happen?


Steve O'Shea
Oct 5th, 2003, 10:55pm
With 'a few years' experience preserving octopus and squid (in museums) I'm used to things going to plan.

Attached is a pic of 'one of those specimens' that you would normally discard in a museum collection, because something 'went wrong'. What has happened is that the animal has gone quite translucent .... generally a sign of poor preservation (whereas all other specimens of this species, preserved during the same trip/expedition, look normal). The hooks on the arms of this small squid (an enoploteuthid) are intact, which means it isn't a problem with formalin solution buffering (they would 'dissolve' otherwise). So what happened? I don't know.

The reason why I ask is that the effect is quite interesting, in that beneath the micoscope the viscera is visible (as are the photophores on the viscera). Now if this procedure could be duplicated, just imagine what systematic characters you might see in larger-bodied squid (if they were treated in exactly the same way). I'd like to duplicate it. The problem is I do not understand the chemistry behind this quirk of preservation. Do we have any chemists out there that have any ideas how we could make larger-bodied squid transparent?

The only chemical that this specimen has been exposed to is formaldehyde; it was fixed in saltwater.
Ta
O

http://www.tonmo.com/phpBB/download.php?id=930

Jean
Oct 6th, 2003, 02:54am
:shock: weird! I wonder about the protein content of the mantle muscle. If it was abnormal ( MUTANT) perhaps it's denatured or something and has thus an odd reaction to the formalin???????????????????????? :bonk: :bugout: :bonk: :bugout: :bonk: :bugout: :bonk: :bugout:

J

PS Keep in mind it took me two goes to get undergrad chemistry by the skin of my teeth

PPS I only did some biochem when I was forced to (screaming and kicking!)

myopsida
Oct 6th, 2003, 05:46am
Steve
Check out the process of clearing & staining vertebrates using sodium borate, KOH, enzymes alizarin etc. etc. on your squids. Could be interesting
(here's a :read: recipe: http://gobiidae.com/methods/method_clear_and_stain.htm

Steve O'Shea
Oct 6th, 2003, 10:10pm
Thanks a million M
Cheers
O

Steve O'Shea
Dec 10th, 2003, 12:50pm
.... we're about to test this recipe; check out the Fossils forum in a month-or-so; exciting things happening! Thanks again M!
O

chrono_war01
Feb 3rd, 2005, 03:18am
any news? :smile:

Infusoria
Feb 3rd, 2005, 06:14am
It would be cool to see the picture. I know you're busy Steve, but any chance of resurrecting it?

Squidman
Feb 3rd, 2005, 09:18pm
Can you imagine an archi or messie that is translucent? If only, if only...

Jean
Feb 5th, 2005, 04:06pm
Can you imagine an archi or messie that is translucent? If only, if only...

ooooooooooooooh yesssssssssssss! :shock:

J